ABSTRACT
Renal disorders have become very common nowadays, which may also lead to kidney failure. The disorder may be caused by the commonly used chemicals such as acetaminophen, CCl4, streptromycin, H2O2 etc. The objective of this work is to determine the nephroprotective potential of ethanolic extract against H2O2 induced toxicity in VERO cell line. Ethanolic extract is known for its antioxidant, anti-inflammatory and anti-microbial effects, which make it a most sought for herbal medicine. Its characteristic features have identified this compound as a potential hepatoprotective and nephroprotective agent. VERO cells are cells taken from the kidney of an African green monkey, which are used in our study. The matured leaves of Melia Azadirachta were used to prepare ethanolic extract and the same was used to test for its inhibitory effect in 96 micro plate formats against in VERO cell lines. To study the cytotoxic properties of ethanolic extract against VERO cell line, we have tested the MTT assay with different concentrations in the range of 1000 to 62.5 μg/ml. From the performed assay, the effect of ethanolic extract drug reveals an enhanced activity on in VERO cell lines and that infers Melia Azadirachta, can be used as nephroprotective agent.
ABSTRACT
The investigation was carried out to determine the qualitative analysis of phytochemical screening and possible chemical components of Mukia maderaspatana (L.) (family: Cucurbitaceae), leaves GC-MS. The plant is an indigenous plant; traditionally it is used as an ingredient of various cocktail preparations and for the management of severe inflammatory disorders in Indian system of medicine. GC-MS analysis of hydroalcoholic extract lead to identification of 7 compounds. This analysis revealed that contains Mukia maderaspatana (L.) leaves mainly Dichloroacetic acid, 4-methylpentyl ester, 2-Butyn-1-ol, 4-methoxy and also showed the presence of other constituents like flavonoids, saponins, carbohydrates, steroids, tannins and phenolic compounds.
ABSTRACT
Doxorubicin (DOX) is a widely used cancer chemotherapeutic agent. However, it generates free oxygen radicals that result in serious dose-limiting cardiotoxicity. Supplementations with Gmelina arborea (Verbenaceae) were proven effective in reducing oxidative stress associated with several ailments. The aim of the current study was to investigate the potential protective effect of Gmelina arborea (GA) against DOX- induced cardiotoxicity in rats. GA was given orally to rats(250&500mg/kg) and DOX (20mg/kg) was administered on the seventh day. GA protected against DOX-induced increased the levels of marker enzymes. It significantly inhibited DOX-provoked glutathione (GSH) depletion in cardiac tissues. The reductions of cardiac activities of catalase (CAT) ,superoxide dismutase (SOD) ,glutathione peroxidase (GSH-Px) and glutathione reductase (GR) were significantly mitigated. Pretreatment of GA significantly guarded against DOX-induced rise of serum lactate dehydrogenase (LDH). GA alleviated histopathological changes in rats’ hearts treated with DOX. In conclusion, GA protects against DOX-induced cardiotoxicity in rats. The study can be attributed, at least in part, to GA’s antioxidant activity.